The Cell Cycle and Cancer Worksheet completed
Introduction To Cellular Biology (BIOL 200)
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Virtual Lab: The Cell Cycle and Cancer Worksheet
In which phase of mitosis do each of the following occur: 1. Centromeres split and chromosomes move toward opposite sides of the cell Anaphase 2. Chromatin coils to form visible chromosomes Prophase
- The nuclear membrane disappears Prophase
- Sister chromatids line up in the center of the cell Metaphase
- In which phases of mitosis are sister chromatids visible, and attached to each other at the centromere? Metaphase
Table 1: Record your data for the number of cells in each stage of the cell cycle observed in normal tissues.
Tissue Type # Cells in Interphase
Telophas e Lung Tissue Sample 1
19 1 0 0 0
Lung Tissue Sample 2
19 0 0 1 0
Total lung 38 1 0 1 0 Stomach Tissue Sample 1
18 0 1 0 1
Stomach Tissue Sample 2
16 1 1 0 2
Total stomach 34 1 2 0 3 Ovarian Tissue Sample 1
19 0 0 1 0
Ovarian Tissue Sample 2
18 0 1 2 0
Total ovarian 37 0 1 3 0
Table 2: Record your data for the number of cells in each stage of the cell cycle observed in cancerous tissues.
Tissue Type # Cells in Interphase
Telophas e Lung Tissue Sample 1
15 1 3 0 1
Lung Tissue Sample 2
16 0 2 1 1
Total lung 31 1 5 1 2 Stomach Tissue Sample 1
14 2 1 1 2
Stomach Tissue Sample 2
13 2 2 2 1
27 4 3 3 3
Ovarian Tissue Sample 1
12 2 1 2 3
Ovarian Tissue Sample 2
11 2 2 3 2
23 4 3 5 5
Table 3: Use the data in Table 1 to calculate the Mitotic Index (average % cells dividing) for each normal tissue type.
Tissue Type Avg. % cells at rest Mitotic Index Lung - normal 15% 5% Stomach - normal 13% 15% Ovary - normal 15% 9%
divide out of control giving the daughter cells the same ability to
divide rapidly.
b. Could you make a prediction about the average % dividing cells in the cancerous breast tissue? Explain your answer.
It is hard to make prediction with out having the normal division
rate and cancerous tissue division rate. But if we have to make
range based on our previous experiments and if the breast cancer
cells have the same pattern as those we have seen in our
experiments, I would say it might be between 12% and 62%
because each of our cancer cells have 10% difference and breast
cells have average cell production.
- Consider the % dividing cells in normal lung, normal stomach, and normal ovarian tissue. Why do you think there are more cells dividing in the stomach and ovary tissue than in the lung tissue?
There are more cells dividing in the stomach and ovary tissue than the lung
tissue because the ovaries and stomach have more production of cells than lung
tissues. The normal lung cells to not have to divide as often because those cells
are not exposed to many things throughout the day. The normal stomach cells
on the other hand divide more because there is a lot of acidity in the stomach
and therefore those cells are exposed to alot of things on a daily basis. As for
the ovaries, there would be a more cell division because that is where new life
is formed and all of the things that come along with that which means that there
should be more division going on.
- In a normal lab setting, we would look at tissue sections from embryonic tissue (blastula stage) of whitefish. How do you think the mitotic index of the blastula tissue compares to that of normal tissue from adult whitefish? Why do you think that?
